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KMID : 0545120190290060913
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 6 p.913 ~ p.922
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Immobilization and Performance of Penicillin G Acylase on Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO Nanocomposites
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Lv Zhixiang
Yu Qingmei
Wang Zhou
Liu Ruijiang
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Abstract
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Magnetic Ni0.7Co0.3Fe2O4 nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites, on which penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and 45oC while immobilized PGA’s a ctivities peaked a t pH 7.5 and 4 5oC. Immobilized PGA had better thermal stability than free PGA at the range of 30-50oC for different time intervals. The activity of free PGA would be 0 and that of immobilized PGA still retained some activities at 60oC after 2 h. Vmax and Km of immobilized PGA were 1.55 mol/min and 0.15 mol/l, respectively. Free PGA’s Vmax and Km separately were 0.74 mol/min and 0.028 mol/l. Immobilized PGA displayed more than 50% activity after 10 successive cycles. We concluded that immobilized PGA with magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites could become a novel example for the immobilization of other amidohydrolases.
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KEYWORD
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Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites, penicillin G acylase, functionalization, surface modification, Immobilization
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